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| CS0790 - Bulletin (93KB) |
| Similar Products |
CS0790
Sigma
Antioxidant Assay Kit
sufficient for 200 tests
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Description
| Application | The kit provides all of the reagents required for an efficient measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates. It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva. |
| General description | The antioxidant assay is based on the formation of a ferryl myoglobin radical from myoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)) to produce a radical cation ABTS+, a soluble green color chromogen that can be determined at 405 nm. In the presence of antioxidants the radical cation is suppressed to an extent dependent on the activity of the antioxidant and the color intensity is decreased proportionally. Trolox™, a water-soluble vitamin E analogue, serves as a standard or a control antioxidant. |
| Legal Information | Trolox is a trademark of Hoffman-LaRoche & Co. AG |
Properties
| usage | sufficient for 200 tests |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Safety
Components
Kit component also available separately
Kit component only
 Description |
|---|
| Hydrogen Peroxide, 3% solution 1mL |
| Myoglobin from horse heart 2X1mg |
| (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2X1mg |
| ABTS, 10 mg tablets 4tabs |
| Phosphate-Citrate Buffer, pH 5 4tabs |
Kit component only
| Description |
|---|
| Assay Buffer 10X 30mL |
| Stop Solution 20mL |
